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The most common approach: 1 ml water in a 5 mg vial 5 mg/ml (5000 mcg/ml) 2 ml water in a 5 mg vial 2.5 mg/ml (2500 mcg/ml) 1 ml water in a 2 mg Semaglutide vial 2 mg/ml a 0.5 mg dose = 25 units (U-100) The exact units to draw depend on your vial size, the water volume, and your dose

ImageJ software (NIH, Bethesda, MD, USA) was used for analysis

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